Genotyping by Sequencing (GBS), which belongs to one of Reduced-Representation Genome Sequencing techniques, is a method to discover single nucleotide polymorphisms (SNP) in order to perform genotyping studies. GBS greatly reduces genome complexity by using restriction enzymes to cleave the DNA coupled with DNA-barcoded adapters. By choosing appropriate restriction enzymes, GBS can provide high SNP coverage in gene-rich regions of the genome in a highly cost-effective manner, and increase the number of tags in the assay. The sequenced portion of the genome is highly consistent within a population because restriction sites are generally conserved across species. This makes the GBS protocol highly suited for experiments that require surveying.
Consequently, GBS is simple, quick, specific, highly reproducible and rapid due to the simultaneous detection of SNPs and genotypes, and may reach important regions of the genome that are inaccessible to capture sequencing approaches. Thus, the key components of this system have the advantages of lower cost, reduced sample handling, fewer PCR and purification steps, no size fractionation, no reference sequence limits, and efficient barcoding, and the system is easy to scale up. These features make GBS a powerful tool for implementing genome-wide association study (GWAS), genomic diversity study, genetic linkage analysis, molecular marker discovery and genomic selection under a large scale of plant breeding programs.
Key Features and Advantages
· Reduced sample handling
· Few PCR & purification steps
· No DNA size fractionation
· Efficient barcoding system
· Simultaneous marker discovery & genotyping
· Large scale data set in less time
Oneomics offers various genotyping services fuelled by state-of - the-art variant detection and SNP genotyping technologies, providing high quality data at low cost per sample. Microarray is a valuable tool for researchers to investigate genetic variants from discovery applications to routine screening, such as single nucleotide polymorphisms (SNPs), and significant structural changes. Affymetrix and Illumina genotyping microarrays both offer positive performance in precision medicine programs, clinical and translational testing, pharmacology, customer screening, and agricultural applications. Multiple genotyping strategies are possible, based on the species and selection of SNPs.
Advantages of SNP Microarray
· Custom, flexible, and scalable
· High call rates (> 99%) and high accuracy
· Cost-effectiveness and high-throughput
· Identifies SNPs in a targeted or whole genome scale
· In addition to SNPs, other genetic differences, such as copy number variations, can be measured
· Applied in biomarker discovery and validation, clinical testing, GWAS, pharmacogenomics, forensics, and breed discrimination
· SNP identification
· Linkage map construction
· Genomic selection
· Population structure analysis
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